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Biacore biacore 2000 instrument
Biacore 2000 Instrument, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biacore+2000+instrument/2000+biacore+instrument/us12622978-234-43-43
Average 86 stars, based on 1 article reviews
biacore 2000 instrument - by Bioz Stars, 2026-09
86/100 stars

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Binding Assay:

Article Title: Recombinant cell surface capture proteins
Article Snippet: .. Measurements to determine binding affinities and kinetic constants were made on a Biacore 2000 instrument. ..

Article Title: Anti-L1CAM antibodies and uses thereof
Article Snippet: .. Typically, the antibody (i) binds with an equilibrium dissociation constant (KD) of approximately less than 10−7 M, such as approximately less than 10−8 M, 10−9 M or 10−10 M or even lower when determined by, e.g., surface plasmon resonance (SPR) technology in a BIACORE® 2000 instrument using the predetermined antigen, e.g., recombinant human MICA or MICB, as the analyte and the antibody as the ligand, or Scatchard analysis of binding of the antibody to antigen positive cells, and (ii) binds to the predetermined antigen with an affinity that is at least two-fold greater than its affinity for binding to a non-specific antigen (e.g., BSA, casein) other than the predetermined antigen or a closely-related antigen. ..

Article Title: Binding agents and uses thereof for central nervous system delivery
Article Snippet: .. Typically, the antibody binds with an equilibrium dissociation constant (KD) of approximately less than 10−6 M, such as approximately less than 10−7, 10−8 M, 10−9 M or 10−10 M or even lower when determined by, e.g., surface plasmon resonance (SPR) technology in a BIACORE 2000 instrument using the target protein as the analyte and the antibody as the binding agent, and binds to the target protein with an affinity that is at least two-fold greater than its affinity for binding to a non-specific target (e.g., BSA, casein). ..

SPR Assay:

Article Title: Anti-L1CAM antibodies and uses thereof
Article Snippet: .. Typically, the antibody (i) binds with an equilibrium dissociation constant (KD) of approximately less than 10−7 M, such as approximately less than 10−8 M, 10−9 M or 10−10 M or even lower when determined by, e.g., surface plasmon resonance (SPR) technology in a BIACORE® 2000 instrument using the predetermined antigen, e.g., recombinant human MICA or MICB, as the analyte and the antibody as the ligand, or Scatchard analysis of binding of the antibody to antigen positive cells, and (ii) binds to the predetermined antigen with an affinity that is at least two-fold greater than its affinity for binding to a non-specific antigen (e.g., BSA, casein) other than the predetermined antigen or a closely-related antigen. ..

Article Title: Anti-met antibodies, bispecific antigen binding molecules that bind met, and methods of use thereof
Article Snippet: .. 20) (maytansinoid B) were determined using a real-time surface plasmon resonance biosensor assay on a Biacore 2000 instrument. .. The Biacore sensor surface was derivatized by amine coupling with a monoclonal mouse anti-human Fc antibody (GE Healthcare, #BR-1008-39) to capture anti-MET ADC and parent unmodified antibodies expressed with human constant regions.

Article Title: Binding agents and uses thereof for central nervous system delivery
Article Snippet: .. Typically, the antibody binds with an equilibrium dissociation constant (KD) of approximately less than 10−6 M, such as approximately less than 10−7, 10−8 M, 10−9 M or 10−10 M or even lower when determined by, e.g., surface plasmon resonance (SPR) technology in a BIACORE 2000 instrument using the target protein as the analyte and the antibody as the binding agent, and binds to the target protein with an affinity that is at least two-fold greater than its affinity for binding to a non-specific target (e.g., BSA, casein). ..

Article Title: Anti-family with sequence similarity 19, member A5 antibodies and method of use thereof
Article Snippet: .. As used herein, an antibody that “binds specifically” to an antigen refers to an antibody that binds to the antigen and substantially identical antigens with high affinity, which means having a KD of 10−7 M or less, preferably 10−8 M or less, even more preferably 10−9 M or less, and most preferably between 10−8 M and 10−10 M or less, when determined by, e.g., immunoassays (e.g., ELISA) or surface plasmon resonance (SPR) technology in a BIACORE 2000 instrument using the predetermined antigen, but does not bind with high affinity to unrelated antigens. ..

Recombinant:

Article Title: Anti-L1CAM antibodies and uses thereof
Article Snippet: .. Typically, the antibody (i) binds with an equilibrium dissociation constant (KD) of approximately less than 10−7 M, such as approximately less than 10−8 M, 10−9 M or 10−10 M or even lower when determined by, e.g., surface plasmon resonance (SPR) technology in a BIACORE® 2000 instrument using the predetermined antigen, e.g., recombinant human MICA or MICB, as the analyte and the antibody as the ligand, or Scatchard analysis of binding of the antibody to antigen positive cells, and (ii) binds to the predetermined antigen with an affinity that is at least two-fold greater than its affinity for binding to a non-specific antigen (e.g., BSA, casein) other than the predetermined antigen or a closely-related antigen. ..

Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance.
Article Snippet: The BIAcore Sensor Chip CM5 (BIAcore, Uppsala, Sweden) was conjugated with a concentration of 1 μg/μL of recombinant MICA protein obtained in 10 mM HEPES pH 7.5, 150 mM NaCl, 0.05% Tween buffer, with a maximum sample volume of 200 μL. .. The analyte (recombinant CRT) was applied at concentrations of 1 nM, 5 nM, and 20 nM using the BIAcore 2000 instrument (BIAcore, Uppsala, Sweden). .. The data were obtained using BIAcore T100 version 2.0 software (BIAcore, Uppsala, Sweden).

Article Title: Calreticulin-Mediated Quality Control of the Non-Classical MHC-I Molecule MICA: Implications for Immune Surveillance
Article Snippet: The BIAcore Sensor Chip CM5 (BIAcore, Uppsala, Sweden) was conjugated with a concentration of 1 μg/μL of recombinant MICA protein obtained in 10 mM HEPES pH 7.5, 150 mM NaCl, 0.05% Tween buffer, with a maximum sample volume of 200 μL. .. The analyte (recombinant CRT) was applied at concentrations of 1 nM, 5 nM, and 20 nM using the BIAcore 2000 instrument (BIAcore, Uppsala, Sweden). .. The data were obtained using BIAcore T100 version 2.0 software (BIAcore, Uppsala, Sweden).

Biosensor Assay:

Article Title: Anti-met antibodies, bispecific antigen binding molecules that bind met, and methods of use thereof
Article Snippet: .. 20) (maytansinoid B) were determined using a real-time surface plasmon resonance biosensor assay on a Biacore 2000 instrument. .. The Biacore sensor surface was derivatized by amine coupling with a monoclonal mouse anti-human Fc antibody (GE Healthcare, #BR-1008-39) to capture anti-MET ADC and parent unmodified antibodies expressed with human constant regions.

Enzyme-linked Immunosorbent Assay:

Article Title: Anti-family with sequence similarity 19, member A5 antibodies and method of use thereof
Article Snippet: .. As used herein, an antibody that “binds specifically” to an antigen refers to an antibody that binds to the antigen and substantially identical antigens with high affinity, which means having a KD of 10−7 M or less, preferably 10−8 M or less, even more preferably 10−9 M or less, and most preferably between 10−8 M and 10−10 M or less, when determined by, e.g., immunoassays (e.g., ELISA) or surface plasmon resonance (SPR) technology in a BIACORE 2000 instrument using the predetermined antigen, but does not bind with high affinity to unrelated antigens. ..

RIA Assay:

Article Title: Claudin-6-specific immunoreceptors and T cell epitopes
Article Snippet: .. Affinities may be readily determined using conventional techniques, such as by equilibrium dialysis; by using the BIAcore 2000 instrument, using general procedures outlined by the manufacturer; by radioimmunoassay using radiolabeled target antigen; or by another method known to the skilled artisan. ..



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